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Protocol Details and Notes |
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You can get the pdf of the 96 plex GBS library preparation protocol here. When you have completed the library preparation, you will need to dilute the libraries prior to submission for sequencing. We are providing a new dilution calculator for samples being clustered onto a flowcell with the TruSeqT SR Cluster Kit v3 protocol. Tell the sequencing facility that the sample is at 2nM and to follow the protocol for denaturation. After that step, have them dilute the sample to 3.6 pM. For maize ApeKI libraries, following these guidelines results in the best data yield for current instruments, chemistry and software versions. For other species / enzymes you will probably need to make some modification of this dilution. If your facility is not using the v3 Cluster Kit please contact
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before you dilute your samples.
This research is supported by a grant from the National Science Foundation.
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