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Institute for Genomic Diversity
151Biotechnology Bldg.
Cornell University
Ithaca, NY 14853
Office Phone 607.254.8470
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Research Focus: Genotyping by Sequencing
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Professional Experience
Sequencing Technology Lead
Buckler Laboratory
Institute for Genomic Diversity
Cornell University
July 2007 to Present.
Responsibilities include development of highly multiplexed next generation sequencing protocols; management, including quality control and tracking of DNA samples from numerous collaborators, coordination and scheduling of data collection and analysis, troubleshooting sequencing protocols, assisting with the development of infrastructure for providing genotypic data to support the research goals of the Maize Diversity Project (Buckler Lab) and other IGD affiliates and collaborators.
Lab Technician III
Scanlon Laboratory
Cornell University
April 2006 to July 2007.
Responsibilities included performing qRT-PCR confirmation of microarray experiments, placing orders, conducting radiation surveys and record keeping, evaluating computer software, managing data, and maintaining the lab web site.
Director, Illinois Genetic Marker Center
University of Illinois at Urbana-Champaign
May 2001 to August 2003
Planned, set up and operated a core molecular genetics laboratory, including optimizing protocols, troubleshooting, designing and implementing an information storage and retrieval system, setting up an accounting system, writing documentation, and designing a training program.
Education
Graduate Program (Non-matriculating), Field of Plant Breeding
August 2003 through August 2005
Cornell University
Master of Science, Natural Resources and Environmental Sciences
May 2001
University of Illinois at Urbana-Champaign
“A Screen for Heat and Low Humidity Tolerance in Cyclamen persicum (Mill.) Seedlings for Use as a Breeding Tool”
Bachelor of Science, Biology
May 1999
University of Illinois at Urbana-Champaign
Associate in Science, Graduation with Honors
December 1996
Parkland College, Champaign Illinois
Publications
RJ Elshire, JC Glaubitz, Q Sun, JA Poland, K Kawamoto, ES Buckler, SE Mitchell. A robust, simple genotyping-by-sequencing (GBS) approach for high diversity species. PLoS ONE 6(5): e19379. doi:10.1371/journal.pone.0019379.
M. Zeid, J.K. Yu, I. Goldowitz, M.E. Denton, Denise E. Costich, C.T. Jayasuriya, M. Saha, R. Elshire, D. Benscher, F. Breseghello, J. Munkvold, R.K. Varshney, G. Belay and M.E. Sorrells (2010) Cross-amplification of EST-derived markers among 16 grass species. Field Crops Research Vol. 118 No. 1:28-35.
Michael A. Gore, Jer-Ming Chia, Robert J. Elshire, Qi Sun, Elhan S. Ersoz, Bonnie L. Hurwitz, Jason A. Peiffer, Michael D. McMullen, George S. Grills, Jeffrey Ross-Ibarra, Doreen H. Ware, Edward S. Buckler (2009) A First-Generation Haplotype Map of Maize. Science 326, 1115
Lionel Brooks, III, Josh Strable, Xiaolan Zhang, Kazuhiro Ohtsu, Ruilian Zhou, Ananda Sarkar, Sarah Hargreaves, Robert J. Elshire, Douglas Eudy, Teresa Pawlowska, Doreen Ware, Diane Janick-Buckner, Brent Buckner, Marja C. P. Timmermans, Patrick S. Schnable, Dan Nettleton, and Michael J. Scanlon (2009) Microdissection of Shoot Meristem Functional Domains. PLoS Genet 5(5): e1000476. doi:10.1371/journal.pgen.1000476
Xiaolan Zhang Shahinez Madi, Lisa Borsuk, Dan Nettleton, Robert J. Elshire, Brent Buckner, Diane Janick-Buckner, Jon Beck, Marja Timmermans, Patrick S. Schnable, Michael J. Scanlon (2007) Laser Microdissection of Narrow Sheath Mutant Maize Uncovers Novel Gene Expression in the Shoot Apical Meristem. PLoS Genet 3(6): e101. doi:10.1371/journal.pgen.0030101
Invited Talks and Workshops
Educational Workshop on Genotyping-by-Sequencing (GBS): presented at the Northeast Regional Life Sciences Core Directors Meeting, Ithaca, NY, November 2011.
Reduced Representation Sequencing for Rapidly Genotyping Highly Diverse Species: presented at Ag Research, Palmerston North, New Zealand, December 2010.
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